技術(shù)原理

         Medimachine II 采用機(jī)械剪切的原理，根據(jù)用戶的不同需要和技術(shù)特性，選擇適當(dāng)?shù)囊淮涡院牟模浜舷鄳?yīng)的操作程序，實(shí)現(xiàn)對(duì)于組織樣品的溫和分離或精細(xì)均質(zhì)化。

• Medimachine II主機(jī)具有兩個(gè)馬達(dá)：低速馬達(dá)最高轉(zhuǎn)速為90rpm，高速馬達(dá)最高轉(zhuǎn)速為22200rpm。
• Medicons / Medicons Max 組織分離器內(nèi)嵌固定的不銹鋼網(wǎng)篩，其上分布有六角形篩孔，每個(gè)篩孔周圍有6個(gè)經(jīng)特殊設(shè)計(jì)的微型刀片以進(jìn)行精細(xì)的組織切割，與主機(jī)的低速馬達(dá)配合可用于動(dòng)物和植物組織細(xì)胞的分離以及從生物實(shí)體組織中制備單細(xì)胞懸液。
• 細(xì)胞懸液經(jīng)不同孔徑的 Filcons / Filcons Max 過(guò)濾器過(guò)濾，完成細(xì)胞分選和純化，用于后續(xù)的流式細(xì)胞分析及細(xì)胞培養(yǎng)等相關(guān)研究與操作。
• FOODcon7 / Prypcons 勻質(zhì)杯配合主機(jī)的高速馬達(dá)使用，快速制備樣品勻漿，實(shí)現(xiàn)組織和細(xì)胞的精細(xì)均質(zhì)化。

產(chǎn)品應(yīng)用/特點(diǎn)

Medimachine II 應(yīng)用

        流式細(xì)胞術(shù)（flow cytometry，F(xiàn)CM）作為一種可在單細(xì)胞水平上對(duì)大量細(xì)胞進(jìn)行快速、準(zhǔn)確、多參數(shù)定量分析和分選的技術(shù)，在細(xì)胞生物學(xué)、免疫學(xué)、腫瘤學(xué)以及臨床診斷分析等方面有著廣泛而深入的應(yīng)用。進(jìn)行FCM分析的前提是單細(xì)胞懸液樣品的制備，此外，單細(xì)胞懸液也是獲得細(xì)胞分離純化、細(xì)胞分析以及細(xì)胞培養(yǎng)可靠結(jié)果的關(guān)鍵先決條件。
          Medimachine II，作為已在生物科研領(lǐng)域被廣泛使用的Medimachine System的全新升級(jí)產(chǎn)品，可在30s～4min內(nèi)完成實(shí)體組織單細(xì)胞懸液樣品的制備，組織細(xì)胞的分離過(guò)程自動(dòng)完成，樣品懸液中活細(xì)胞比例高（cell viability，在程序正確完成的情況下可達(dá)到80%），適合進(jìn)行FCM分析、活體組織檢查以及相關(guān)細(xì)胞的篩選、純化和培養(yǎng)。
         此外，Medimachine II還具備對(duì)生物樣品的精細(xì)均質(zhì)化功能，產(chǎn)物可用于核酸提取、DNA擴(kuò)增等分子生物學(xué)研究以及化學(xué)殘留物、病毒和細(xì)菌的檢測(cè)分析等。

單細(xì)胞懸液制備&精細(xì)勻質(zhì)--雙重模式

         Medimachine II將生物樣品的單細(xì)胞懸液制備與均質(zhì)化處理兩種功能整合于一體，極大地方便了用戶的不同需求。

核心應(yīng)用

·流式細(xì)胞分析
·細(xì)胞分離與純化
·細(xì)胞培養(yǎng)
·活體組織檢查
·核酸提取與DNA擴(kuò)增
·化學(xué)殘留物、病毒和細(xì)菌分析等

性能特點(diǎn)

·具有交互式觸摸屏，可戴實(shí)驗(yàn)室手套觸控操作。
·軟件提供有操作模式和操作咨詢功能，并有多種語(yǔ)言可供設(shè)置。
·具有手動(dòng)和自動(dòng)兩種模式：手動(dòng)模式下，可自主設(shè)定運(yùn)行時(shí)間；自動(dòng)模式下，直接選定時(shí)間選項(xiàng)。
·具有兩個(gè)馬達(dá)：低速馬達(dá)最高轉(zhuǎn)速為90rpm，實(shí)現(xiàn)單細(xì)胞分離；高速馬達(dá)最高轉(zhuǎn)速為22,200rpm，實(shí)現(xiàn)組織均質(zhì)化。
·程序運(yùn)行結(jié)束時(shí)，馬達(dá)會(huì)自動(dòng)停止，上蓋自動(dòng)解鎖。
·程序運(yùn)行期間，用戶根據(jù)需要可以控制終止運(yùn)行。
·用戶可根據(jù)需要添加或刪除新的自定義程序。
·具有多種顏色的機(jī)身效果可供設(shè)置。

耗材

Medicons組織分離器（1 ml） ·無(wú)菌包裝，35μm篩孔孔徑 ·無(wú)菌包裝，50μm篩孔孔徑 ·普通包裝，35μm篩孔孔徑 ·普通包裝，50μm篩孔孔徑
Medicons Max組織分離器（16 ml ） ·無(wú)菌包裝，50μm篩孔孔徑 ·普通包裝，50μm篩孔孔徑
Filcons細(xì)胞過(guò)濾器（濾膜面積80mm2） -可與5ml Falcon離心管配套使用，離心管連接于流式細(xì)胞儀上，執(zhí)行后續(xù)操作。 ·無(wú)菌包裝 ·10μm濾膜孔徑（棕） ·20μm濾膜孔徑（紅） ·30μm濾膜孔徑（橙） ·50μm濾膜孔徑（綠） ·70μm濾膜孔徑（紫） ·100μm濾膜孔徑（黑） ·200μm濾膜孔徑（白） ·500μm濾膜孔徑（藍(lán)） ·杯型（適用5ml以下細(xì)胞懸液的過(guò)濾） ·10μm濾膜孔徑（棕） ·20μm濾膜孔徑（紅） ·30μm濾膜孔徑（橙） ·50μm濾膜孔徑（綠） ·70μm濾膜孔徑（紫） ·100μm濾膜孔徑（黑） ·200μm濾膜孔徑（白） ·500μm濾膜孔徑（藍(lán)） ·普通包裝 ·柱型（適用5ml以上細(xì)胞懸液的過(guò)濾） ·10μm濾膜孔徑（棕） ·20μm濾膜孔徑（紅） ·30μm濾膜孔徑（橙） ·50μm濾膜孔徑（綠） ·70μm濾膜孔徑（紫） ·100μm濾膜孔徑（黑） ·200μm濾膜孔徑（白） ·500μm濾膜孔徑（藍(lán)） ·杯型（適用5ml以下細(xì)胞懸液的過(guò)濾） ·10μm濾膜孔徑（棕） ·20μm濾膜孔徑（紅） ·30μm濾膜孔徑（橙） ·50μm濾膜孔徑（綠） ·70μm濾膜孔徑（紫） ·100μm濾膜孔徑（黑） ·200μm濾膜孔徑（白） ·500μm濾膜孔徑（藍(lán)）
Filcons Max細(xì)胞過(guò)濾器（濾膜面積660 mm2） -可與50ml Falcon錐形離心管或同類型50ml離心管配套使用。 ·無(wú)菌包裝 ·杯型 ·30μm濾膜孔徑（橙） ·50μm濾膜孔徑（綠） ·70μm濾膜孔徑（紫） ·100μm濾膜孔徑（黑） ·普通包裝 ·杯型 ·30μm濾膜孔徑（橙） ·50μm濾膜孔徑（綠） ·70μm濾膜孔徑（紫） ·100μm濾膜孔徑（黑）
FOODcon勻質(zhì)樣品杯 ·最多可加入25?ml樣品
Prypcons勻質(zhì)樣品杯 ·最多可加入6 ml樣品

產(chǎn)品規(guī)格

型號(hào) Medimachine II 認(rèn)證標(biāo)準(zhǔn) CE認(rèn)證；符合UL / CSA 尺寸 長(zhǎng)×寬×高 211 mm x 164 mm x 257 mm 重量 2.50 kg 主電源 功耗 120W 電源電壓 85～264 VAC， 47-63 Hz 保險(xiǎn)絲 2 x 2A 馬達(dá) 低速規(guī)格 轉(zhuǎn)速 - > 90 rpm 旋轉(zhuǎn)方向 - > 順時(shí)針 最大扭矩 - > 420 mNm 高速規(guī)格 轉(zhuǎn)速 - > 22,200 rpm 旋轉(zhuǎn)方向 - > 順時(shí)針 失速轉(zhuǎn)矩 - > 123 mNm 操作條件 工作溫度 +5到+45℃ 濕度 低于90％ 海拔高度 無(wú)要求 儲(chǔ)存溫度 0 到+50°C

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