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品牌介紹

       活體體內(nèi)光學成像(Optical in vivo Imaging)主要采用生物發(fā)光(Biolumininescence)與熒光(Fluorescence)兩種技術(shù)標記細胞或者DNA,利用非常靈敏的光學 檢測儀器,直接檢測活體生物體內(nèi)的細胞活動和基因行為。這項技術(shù)具有操作簡單、所得結(jié)果直觀、靈敏度高等特點,因為已經(jīng)廣泛應(yīng)用于生命科學,醫(yī)學研究以及藥物開發(fā)等發(fā)面。該技術(shù)應(yīng)用于植物研究方面,具體的應(yīng)用領(lǐng)域包括:植物的突變體篩選、植物抗逆性狀研究、離體細胞的基因表達、植物生理節(jié)律研究、植物發(fā)育研究、蛋白互作等。 

技術(shù)簡介 

       Lumazone系統(tǒng)是美國Princeton Instrument(PI, Roper Technologies, Ins. 集團子公司)研制的活體生物發(fā)光系統(tǒng),可以觀察并成像將化學發(fā)光或者熒光標記后的動物、植物和微生物整體標本,并結(jié)合專業(yè)的數(shù)據(jù)分析軟件,完成動物和植物體內(nèi)基因表達的原位跟蹤,藥物篩選和藥效檢測,植物突變體檢測和篩選,以及單細胞內(nèi)的基因表達等研究。     

      在植物活體分子影像學的研究過程中,美國Roper公司最早參與其中,為科學家提供了高靈敏度的成像系統(tǒng)。1997年,朱健康教授在Plant Cell上發(fā)表的文章就已經(jīng)使用Roper公司生產(chǎn)的高靈敏度 CCD。北京博益?zhèn)I(yè)儀器有限公司,作為 Roper Scientific公司在中國的代理,一直致力于將其最佳性能的光學成像系統(tǒng)推薦給客戶,并憑借在生命科學研究領(lǐng)域多年來積累的經(jīng)驗,能夠根據(jù)客戶需求為客戶量身定制成像系統(tǒng),因此,已成為在此領(lǐng)域中具領(lǐng)先地位的系統(tǒng)供應(yīng)商之一。

系統(tǒng)配置

     >> 高性能CCD相機

      >> 多功能成像暗箱
      >> 專用成像鏡頭
      >> 熒光光源(選配)
      >> 專用濾光片(選配)
      >> 專業(yè)成像控制和圖像處理分析軟件

      >> 專用工作站及顯示器

      >> 暗箱運輸箱

產(chǎn)品型號

      近年來,為了滿足植物學研究的需要,解決植物活體熒光成像時葉綠體熒光干擾的問題,博益公司推出了多款專業(yè)型的植物整體分子影像系統(tǒng),包括最新的Lumazone Sophia 2048B、 Lumazone Pylon 1300B、Lumazone Pylon2408B 等,并針對不同實驗需求配置突變體篩選暗箱或者光周期暗箱。實驗無需將植物樣本做任何處理,并可通過生物發(fā)光(LUC)和熒光方法實時觀察植物樣本基因表達的變化,準確定量分析。Lumazone 產(chǎn)品根據(jù)可選CCD型號以及暗箱功能的不同,系統(tǒng)型號如下表。

CCD型號

突變體篩選系統(tǒng) 光周期成像系統(tǒng) 熒光成像系統(tǒng)
Pixis 1024B Lumazone 1024B Lumazone 1024B Light Lumaozne 1204B FL
Pixis 2048B Lumazone 2048B
Lumazone 2048B Light
Lumaozne 2048B FL
Sophia 2048B

Lumaozne Sophia 2048B

Lumaozne Sophia 2048B Light
Lumaozne Sophia 2048B FL
Pylon 1300B

Lumazone Pylon 1300B

Lumaone Pylon 1300B Light
Lumazone Pylon 1300B FL
Pylon 2048B LumazonPylon 2048B
LumazonPylon 2048B Light
LumazonPylon 2048B FL
Lumo Lumazone Lumo

Lumazone Lumo Light

Lumazoen Lumo FL

Lumazone系統(tǒng)參數(shù)


典型應(yīng)用案例

>> 突變體篩選                                                                            >> 逆境處理研究       

        


>> 蛋白互作研究                                                                          >> 基因誘導表達研究


           


 >> 節(jié)律相關(guān)研究                                                                       >> 甲基化研究     


         

發(fā)表文獻

RNA helicase-like protein as an early  regulator  of  transcription factors for plant chilling and freezing tolerance

PNAS 9911507–11512August 20, 2002

Arabidopsis hot Mutants Define Multiple Functions Required for Acclimation to High Temperatures

Plant Physiology 132757–767  Jun 2003

Screening for Gene Regulation Mutants by Bioluminescence 

Imaging

Sci. STKE 140, pl10. (9 July 2002)

Flagellin induces innate immunity in nonhost interactions that is suppressed by Pseudomonas syringae effectors

PNAS 10212990–12995Sep 2005

Arabidopsis Protein Kinase PKS5 Inhibits the Plasma Membrane 

H1-ATPase by Preventing Interaction with 14-3-3 Protein

PLANT CELL 19: 1617 - 1634.( May 2007

Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants

Plant Physiology 146: 368 - 376.( Feb 2008

C-terminal domain phosphatase-like family members (AtCPLs) differentially regulate Arabidopsis thaliana abiotic 

stress signaling, growth, and development

PNAS 99 10893–10898 Aug 2002 

RAR1, a central player in plant immunity, is targeted by Pseudomonas syringae effector AvrB

PNAS 10319200–19205Dec 2006

STABILIZED1, a Stress-Upregulated Nuclear Protein, Is Required for Pre-mRNA Splicing, mRNA Turnover, and 

Stress Tolerance in Arabidopsis

The Plant Cell 181736–1749  Jul 2006

FIONA1 Is Essential for Regulating Period Length in the Arabidopsis Circadian Clock

The Plant Cell 20: 307–319,(Feb 2008)

The Plant Cell 20: 307–319,Feb 2008

Interaction of Osmotic Stress, Temperature, and Abscisic Acid in the Regulation of Gene Expression in Arabidopsis

Plant Physiology    119 205–211 Jan 1999

Regulated Expression of Arabidopsis Phosphate Transporters1

Plant Physiology 130 221–233  Sep 2002

Regulation   of   Osmotic  Stress-responsive  Gene  Expression  

by   the LOS6/ABA1 Locus in Arabidopsis

THE JOURNAL OF BIOLOGICAL CHEMISTRY 2778588–8596 

Mar 2002

A conserved transcriptional regulator is required for RNA-directed DNA methylation and plant development

Genes Dev. 23: 2717-2722Oct 2009

FIERY1 encoding an inositol polyphosphate 1-phosphatase  is anegative regulator of abscisic acid and stress signaling in 

Arabidopsis

Genes & Dev 15: 1971-1984Jul 2001

A DEAD Box RNA Helicase Is Essential for mRNA Export and Important for Development and Stress Responsesin Arabidopsis

The Plant Cell 17256–267Jan    2005

Genetic Analysis of Osmotic and Cold Stress Signal Transduction 

in Arabidopsis: lnteractions and Convergence of Abscisic Acid-Dependent and Abscisic Acid-lndependent Pathways

The Plant Cell 91935-1949,  Nov 1997

A Peroxidase Contributes to ROS Production during Arabidopsis Root Response to Potassium

Deficiency

Molecular Plant    3420–427  Mar 2010

Activation of a COl1-dependent pathway in Arabidopsis 

by Pseudomonas syringae typeIII effectors and coronatine

The Plant Journal    37589-602  Jan 2004

A Putative Arabidopsis Nucleoporin, AtNUP160, Is Critical for RNA Export and Required for Plant Tolerance to Cold Stress

MOLECULAR AND CELLULAR BIOLOGY 269533–9543 Dec.2006

Regulated Expression of Arabidopsis Phosphate Transporters

Plant Physiology 130: 221 - 233  Sep 2002

COP1-Mediated Ubiquitination of CONSTANS Is Implicated in Cryptochrome Regulation of Flowering in Arabidopsis

The Plant Cell, Vol. 20: 292–306, February 2008

Ethanol breaks dormancy of the potato tuber apical bud

Journal of Experimental Botany 562515–2525Sep 2005

Disruption of Arabidopsis CHY1 Reveals an Important Role of Metabolic Status in Plant Cold

Molecular Plant    259–72  January 2009

HOS10 encodes an R2R3-type MYB transcription factor essential for cold acclimation in plants

PNAS 1029966–9971  Jul 12, 2005

Involvement of Arabidopsis HOS15 in histone deacetylation and cold tolerance

PNAS 1054945–4950  Mar 2008

NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V 

and is required for RNA-directed DNA methylation

GENES & DEVELOPMENT 23:318–330Jan 2009

Role of the Arabidopsis DNA glycosylase_lyase ROS1 in active DNA demethylation

PNAS 10311796–11801Aug 2006  

A GUS/Luciferase Fusion Reporter for Plant Gene Trapping and for Assay of Promoter Activity with Luciferin-Dependent Control of the Reporter Protein Stability

Plant Cell Physiol. 48(8): 1121–1131 (2007)

Isolation and Characterization of cul1-7, a Recessive Allele of CULLIN1 That Disrupts SCF Function at the C Terminus of CUL1 in Arabidopsis thaliana

Genetics 181: 945–963 (March 2009)

The Plant Cuticle Is Required for Osmotic Stress Regulation of Abscisic Acid Biosynthesis and Osmotic Stress Tolerance in Arabidopsis

PLANT CELL, May 2011; 23: 1971 - 1984

Testing Time: Can Ethanol-Induced Pulses of Proposed Oscillator Components Phase Shift Rhythms in Arabidopsis?

JOURNAL OF BIOLOGICAL RHYTHMS, Vol. 23 No. 6, December 2008 463-471

Fully Codon-Optimized luciferase Uncovers Novel Temperature Characteristics of the Neurospora Clock

EUKARYOTIC CELL, Jan. 2008, p. 28–37

RTE1 Is a Golgi-Associated and ETR1-DependentNegative Regulator of Ethylene Responses

Plant Physiology, September 2007, Vol. 145, pp. 75–86


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